PUBLICATION

Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm

Authors
Perens, E.A., Garavito-Aguilar, Z.V., Guio-Vega, G.P., Peña, K.T., Schindler, Y.L., Yelon, D.
ID
ZDB-PUB-161103-5
Date
2016
Source
eLIFE   5: (Journal)
Registered Authors
Perens, Elliot, Yelon, Deborah
Keywords
developmental biology, stem cells, zebrafish
MeSH Terms
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism
  • Body Patterning/genetics
  • Cell Differentiation/genetics
  • Gene Expression Regulation, Developmental
  • Mesoderm/growth & development
  • Mesoderm/metabolism
  • Mutation
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Veins/growth & development
  • Veins/metabolism*
  • Organogenesis/genetics
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Kidney/growth & development
  • Kidney/metabolism*
(all 20)
PubMed
27805568 Full text @ Elife
Abstract
Proper organogenesis depends upon defining the precise dimensions of organ progenitor territories. Kidney progenitors originate within the intermediate mesoderm (IM), but the pathways that set the boundaries of the IM are poorly understood. Here, we show that the bHLH transcription factor Hand2 limits the size of the embryonic kidney by restricting IM dimensions. The IM is expanded in zebrafish hand2 mutants and is diminished when hand2 is overexpressed. Within the posterior mesoderm, hand2 is expressed laterally adjacent to the IM. Venous progenitors arise between these two territories, and hand2 promotes venous development while inhibiting IM formation at this interface. Furthermore, hand2 and the co-expressed zinc-finger transcription factor osr1 have functionally antagonistic influences on kidney development. Together, our data suggest that hand2 functions in opposition to osr1 to balance the formation of kidney and vein progenitors by regulating cell fate decisions at the lateral boundary of the IM.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci1TgTransgenic Insertion
    pd24TgTransgenic Insertion
      s6
        		Deficiency
        s896TgTransgenic Insertion
          sd28TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            hand2MO2-hand2MRPHLNO
            osr1MO1-osr1MRPHLNO
            tp53MO4-tp53MRPHLNO
            1 - 3 of 3
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            Fish
            No data available
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            mCherryEFGmCherry
            1 - 2 of 2
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            Mapping
            No data available
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            Citation
            Perens, E.A., Garavito-Aguilar, Z.V., Guio-Vega, G.P., Peña, K.T., Schindler, Y.L., Yelon, D. (2016) Hand2 inhibits kidney specification while promoting vein formation within the posterior mesoderm. eLIFE. 5.