PUBLICATION
Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish
- Authors
- Windner, S.E., Doris, R.A., Ferguson, C.M., Nelson, A.C., Valentin, G., Tan, H., Oates, A.C., Wardle, F.C., Devoto, S.H.
- ID
- ZDB-PUB-150301-4
- Date
- 2015
- Source
- Development (Cambridge, England) 142(6): 1159-68 (Journal)
- Registered Authors
- Devoto, Stephen Henri, Nelson, Andrew, Oates, Andrew, Wardle, Fiona
- Keywords
- none
- Datasets
- GEO:GSE57332
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Antibodies, Monoclonal
- Base Sequence
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Chromatin Immunoprecipitation
- Gene Expression Regulation, Developmental/genetics
- Gene Expression Regulation, Developmental/physiology*
- Gene Knockdown Techniques
- Gene Regulatory Networks/genetics
- Gene Regulatory Networks/physiology
- Immunohistochemistry
- In Situ Hybridization
- Molecular Sequence Data
- Morpholinos/genetics
- Muscle Development/physiology*
- Muscle, Skeletal/embryology*
- Nuclear Proteins/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Somites/embryology
- T-Box Domain Proteins/immunology
- T-Box Domain Proteins/metabolism*
- Zebrafish/embryology*
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism*
- PubMed
- 25725067 Full text @ Development
Citation
Windner, S.E., Doris, R.A., Ferguson, C.M., Nelson, A.C., Valentin, G., Tan, H., Oates, A.C., Wardle, F.C., Devoto, S.H. (2015) Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish. Development (Cambridge, England). 142(6):1159-68.
Abstract
During embryonic development, the paraxial mesoderm becomes segmented into somites, within which proliferative muscle progenitors and muscle fibers establish the skeletal musculature. Here, we demonstrate that a gene network previously implicated in somite boundary formation, involving the transcriptional regulators Tbx6, Mesp-b and Ripply1, also confers spatial and temporal regulation to skeletal myogenesis in zebrafish. We show that Tbx6 directly regulates mesp-b and ripply1 expression in vivo, and that the interactions within the regulatory network are largely conserved among vertebrates. Mesp-b is necessary and sufficient for the specification of a subpopulation of muscle progenitors, the central proportion of the Pax3(+)/Pax7(+) dermomyotome. Conditional ubiquitous expression indicates that Mesp-b acts by inhibiting myogenic differentiation and by inducing the dermomyotome marker meox1. By contrast, Ripply1 induces a negative-feedback loop by promoting Tbx6 protein degradation. Persistent Tbx6 expression in Ripply1 knockdown embryos correlates with a deficit in dermomyotome and myotome marker gene expression, suggesting that Ripply1 promotes myogenesis by terminating Tbx6-dependent inhibition of myogenic maturation. Together, our data suggest that Mesp-b is an intrinsic upstream regulator of skeletal muscle progenitors and that, in zebrafish, the genes regulating somite boundary formation also regulate the development of the dermomyotome in the anterior somite compartment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping