ZFIN ID: ZDB-PUB-150301-4
Tbx6, Mesp-b and Ripply1 regulate the onset of skeletal myogenesis in zebrafish
Windner, S.E., Doris, R.A., Ferguson, C.M., Nelson, A.C., Valentin, G., Tan, H., Oates, A.C., Wardle, F.C., Devoto, S.H.
Date: 2015
Source: Development (Cambridge, England)   142(6): 1159-68 (Journal)
Registered Authors: Devoto, Stephen Henri, Nelson, Andrew, Oates, Andrew, Wardle, Fiona
Keywords: none
Microarrays: GEO:GSE57332
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Antibodies, Monoclonal
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Chromatin Immunoprecipitation
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Gene Knockdown Techniques
  • Gene Regulatory Networks/genetics
  • Gene Regulatory Networks/physiology
  • Immunohistochemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Morpholinos/genetics
  • Muscle Development/physiology*
  • Muscle, Skeletal/embryology*
  • Nuclear Proteins/metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Somites/embryology
  • T-Box Domain Proteins/immunology
  • T-Box Domain Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish Proteins/immunology
  • Zebrafish Proteins/metabolism*
PubMed: 25725067 Full text @ Development
During embryonic development, the paraxial mesoderm becomes segmented into somites, within which proliferative muscle progenitors and muscle fibers establish the skeletal musculature. Here, we demonstrate that a gene network previously implicated in somite boundary formation, involving the transcriptional regulators Tbx6, Mesp-b and Ripply1, also confers spatial and temporal regulation to skeletal myogenesis in zebrafish. We show that Tbx6 directly regulates mesp-b and ripply1 expression in vivo, and that the interactions within the regulatory network are largely conserved among vertebrates. Mesp-b is necessary and sufficient for the specification of a subpopulation of muscle progenitors, the central proportion of the Pax3(+)/Pax7(+) dermomyotome. Conditional ubiquitous expression indicates that Mesp-b acts by inhibiting myogenic differentiation and by inducing the dermomyotome marker meox1. By contrast, Ripply1 induces a negative-feedback loop by promoting Tbx6 protein degradation. Persistent Tbx6 expression in Ripply1 knockdown embryos correlates with a deficit in dermomyotome and myotome marker gene expression, suggesting that Ripply1 promotes myogenesis by terminating Tbx6-dependent inhibition of myogenic maturation. Together, our data suggest that Mesp-b is an intrinsic upstream regulator of skeletal muscle progenitors and that, in zebrafish, the genes regulating somite boundary formation also regulate the development of the dermomyotome in the anterior somite compartment.