PUBLICATION
Optimized cell transplantation using adult rag2 mutant zebrafish
- Authors
- Tang, Q., Abdelfattah, N.S., Blackburn, J.S., Moore, J.C., Martinez, S.A., Moore, F.E., Lobbardi, R., Tenente, I.M., Ignatius, M.S., Berman, J.N., Liwski, R.S., Houvras, Y., Langenau, D.M.
- ID
- ZDB-PUB-140722-20
- Date
- 2014
- Source
- Nature Methods 11(8): 821-4 (Journal)
- Registered Authors
- Berman, Jason, Houvras, Yariv, Ignatius, Myron, Langenau, David, Moore, John
- Keywords
- none
- MeSH Terms
-
- Aged
- Animals
- Cell Transplantation*
- DNA-Binding Proteins/genetics*
- Humans
- Mutation*
- Zebrafish/genetics*
- PubMed
- 25042784 Full text @ Nat. Methods
Citation
Tang, Q., Abdelfattah, N.S., Blackburn, J.S., Moore, J.C., Martinez, S.A., Moore, F.E., Lobbardi, R., Tenente, I.M., Ignatius, M.S., Berman, J.N., Liwski, R.S., Houvras, Y., Langenau, D.M. (2014) Optimized cell transplantation using adult rag2 mutant zebrafish. Nature Methods. 11(8):821-4.
Abstract
Cell transplantation into adult zebrafish has lagged behind mouse models owing to the lack of immunocompromised strains. Here we have created rag2(E450fs) mutant zebrafish that have reduced numbers of functional T and B cells but are viable and fecund. Mutant fish engraft muscle, blood stem cells and various cancers. rag2(E450fs) mutant zebrafish are the first immunocompromised zebrafish model that permits robust, long-term engraftment of multiple tissues and cancer.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping