PUBLICATION

Bioelectric signaling regulates size in zebrafish fins

Authors
Perathoner, S., Daane, J.M., Henrion, U., Seebohm, G., Higdon, C.W., Johnson, S.L., Nüsslein-Volhard, C., and Harris, M.P.
ID
ZDB-PUB-140321-22
Date
2014
Source
PLoS Genetics   10(1): e1004080 (Journal)
Registered Authors
Harris, Matthew, Johnson, Stephen L., Nüsslein-Volhard, Christiane
Keywords
Potassium channels, Fishes, Zebrafish, Membrane potential, Xenopus oocytes, Hyperpolarization, Cloning, Phenotypes
MeSH Terms
  • Animal Fins/growth & development*
  • Animals
  • Mutation
  • Potassium/metabolism
  • Potassium Channels/genetics*
  • Potassium Channels/metabolism
  • Signal Transduction*
  • Xenopus
  • Zebrafish/genetics*
  • Zebrafish/growth & development
PubMed
24453984 Full text @ PLoS Genet.
Abstract

The scaling relationship between the size of an appendage or organ and that of the body as a whole is tightly regulated during animal development. If a structure grows at a different rate than the rest of the body, this process is termed allometric growth. The zebrafish another longfin (alf) mutant shows allometric growth resulting in proportionally enlarged fins and barbels. We took advantage of this mutant to study the regulation of size in vertebrates. Here, we show that alf mutants carry gain-of-function mutations in kcnk5b, a gene encoding a two-pore domain potassium (K+) channel. Electrophysiological analysis in Xenopus oocytes reveals that these mutations cause an increase in K+ conductance of the channel and lead to hyperpolarization of the cell. Further, somatic transgenesis experiments indicate that kcnk5b acts locally within the mesenchyme of fins and barbels to specify appendage size. Finally, we show that the channel requires the ability to conduct K+ ions to increase the size of these structures. Our results provide evidence for a role of bioelectric signaling through K+ channels in the regulation of allometric scaling and coordination of growth in the zebrafish.

Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes