PUBLICATION

Analysis of post-embryonic heart development and maturation in the zebrafish, Danio rerio

Authors
Singleman, C., and Holtzman, N.G.
ID
ZDB-PUB-121030-7
Date
2012
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   241(12): 1993-2004 (Journal)
Registered Authors
Holtzman, Nathalia Glickman
Keywords
heart development, cardiac maturation, zebrafish, danio rerio, ventricle, atrium, trabeculation, bulbous ateriosus, congenital heart disease, myh6, weak atrium
MeSH Terms
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Heart Defects, Congenital/embryology
  • Heart Defects, Congenital/etiology
  • Heart Defects, Congenital/genetics
  • Heart Ventricles/cytology
  • Heart Ventricles/embryology*
  • Heart Atria/embryology
  • Myocardium/cytology
  • Myocardium/metabolism*
  • Myosin Heavy Chains/genetics
  • Myosin Heavy Chains/metabolism
  • Mutation
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Animals
(all 16)
PubMed
23074141 Full text @ Dev. Dyn.
Abstract

Background

Cardiac maturation is vital for animal survival and must occur throughout the animal's life. Zebrafish are increasingly used to model cardiac disease; however, little is known about how the cardiovascular system matures. We conducted a systematic analysis of cardiac maturation from larvae though to adulthood and assessed cardiac features influenced by genetic and environmental factors.

Results

We identified a novel step in cardiac maturation, termed cardiac rotation, where the larval heart rotates into its final orientation within the thoracic cavity with the atrium placed behind the ventricle. This rotation is followed by linear ventricle growth and an increase in the angle between bulbous arteriosus and the ventricle. The ventricle transitions from a rectangle, to a triangle and ultimately a circle that is significantly enveloped by the atrium. In addition, trabeculae are similarly patterned in the zebrafish and humans with both muscular fingerlike projections and muscle bands that span the cardiac chamber. Interestingly, partial loss of atrial contraction in myosin heavy chain 6 (myh6/weahu423/+) mutants result in the adult maintaining a larval cardiac form.

Conclusions

These findings serve as a foundation for the study of defects in cardiovascular development from both genetic and environmental factors.

Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
myh6dhu423/+standard conditionsProtruding-mouth
myh6dhu423/+standard conditionsProtruding-mouth
myh6dhu423/+standard conditionsAdult
myh6dhu423/+standard conditionsAdult
myh6dhu423/+standard conditionsAdult
myh6dhu423/+standard conditionsAdult
myh6dhu423/+standard conditionsAdult
myh6dhu423/+standard conditionsAdult
1 - 8 of 8
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
dhu423
    		Point Mutation
    f2TgTransgenic Insertion
      twu34TgTransgenic Insertion
        y1TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          f2Tg; twu34Tg
          f2Tg; y1Tg
          myh6dhu423/+
          twu34Tg
          WT
          y1Tg
          1 - 6 of 6
          Show
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          DsRed2EFGDsRed2
          EGFPEFGEGFP
          1 - 2 of 2
          Show
          Mapping
          No data available
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          Citation
          Singleman, C., and Holtzman, N.G. (2012) Analysis of post-embryonic heart development and maturation in the zebrafish, Danio rerio. Developmental Dynamics : an official publication of the American Association of Anatomists. 241(12):1993-2004.