ZFIN ID: ZDB-PERS-970217-7
Holtzman, Nathalia Glickman
Email: nathalia.holtzman@qc.cuny.edu
URL: http://qcpages.qc.edu/Biology/Holtzman/Holtzman.html
Affiliation: Holtzman Lab
Address: Queens College SB D328 65-30 Kissena Blv Flushing, NY 11367 USA
Country: United States
Phone: 718-997-3678
Fax: 718-997-3445

During heart formation, cardiac precursors start out as bilateral sheets of cells that move toward the midline, where they converge, surround the central endocardium, and rearrange to create the heart tube. Though the tissue level shape changes that occur during heart tube formation are beginning to be understood, little is known about the cell behaviors or the molecular regulation of this process. Tracking the movement of individual GFP-expressing cardiomyocytes in wild-type zebrafish embryos and in zebrafish mutants with heart tube defects suggests that highly regulated, coordinated cell movements are required to form the heart.

We are currently examining the role of the endoderm, endocardium and yolk in directing the movement of the myocardium.

Singleman, C., Holtzman, N.G. (2021) PCB and TCDD derived embryonic cardiac defects result from a novel AhR pathway. Aquatic toxicology (Amsterdam, Netherlands). 233:105794
Singleman, C., Zimmerman, A., Harrison, E., Roy, N., Wirgin, I., Holtzman, N.G. (2020) Toxic Effects of PCB Congeners and Aroclors on Embryonic Growth and Development. Environmental toxicology and chemistry. 40(1):187-201
Prabhudesai, S., Bensabeur, F.Z., Abdullah, R., Basak, I., Baez, S., Alves, G., Holtzman, N.G., Larsen, J.P., Møller, S.G. (2016) LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. Journal of neuroscience research. 94(8):717-35
Holtzman, N.G., Iovine, M.K., Liang, J.O., Morris, J. (2016) Learning to Fish with Genetics: A Primer on the Vertebrate Model Danio rerio. Genetics. 203:1069-1089
Arif Pavel, M., Lv, C., Ng, C., Yang, L., Kashyap, P., Lam, C., Valentino, V., Fung, H.Y., Campbell, T., Møller, S.G., Zenisek, D., Holtzman, N.G., Yu, Y. (2016) Function and regulation of TRPP2 ion channel revealed by a gain-of-function mutant. Proceedings of the National Academy of Sciences of the United States of America. 113(17):E2363-72
Singleman, C., Holtzman, N.G. (2014) Growth and Maturation in the Zebrafish, Danio Rerio: A Staging Tool for Teaching and Research. Zebrafish. 11(4):396-406
Lenhart, K.F., Holtzman, N.G., Williams, J.R., and Burdine, R.D. (2013) Integration of Nodal and BMP Signals in the Heart Requires FoxH1 to Create Left-Right Differences in Cell Migration Rates That Direct Cardiac Asymmetry. PLoS Genetics. 9(1):e1003109
Singleman, C., and Holtzman, N.G. (2012) Analysis of post-embryonic heart development and maturation in the zebrafish, Danio rerio. Developmental dynamics : an official publication of the American Association of Anatomists. 241(12):1993-2004
Singleman, C., and Holtzman, N.G. (2011) Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio. Journal of visualized experiments : JoVE.
Osborne, N., Brand-Arzamendi, K., Ober, E.A., Jin, S.W., Verkade, H., Holtzman, N.G., Yelon, D., and Stainier, D.Y. (2008) The spinster homolog, two of hearts, is required for sphingosine 1-phosphate signaling in zebrafish. Current biology : CB. 18(23):1882-1888
de Campos-Baptista, M.I., Holtzman, N.G., Yelon, D., and Schier, A.F. (2008) Nodal signaling promotes the speed and directional movement of cardiomyocytes in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(12):3624-3633
Baker, K., Holtzman, N.G., and Burdine, R.D. (2008) Direct and indirect roles for Nodal signaling in two axis conversions during asymmetric morphogenesis of the zebrafish heart. Proceedings of the National Academy of Sciences of the United States of America. 105(37):13924-13929
Holtzman, N.G., Schoenebeck, J.J., Tsai, H.J., and Yelon, D. (2007) Endocardium is necessary for cardiomyocyte movement during heart tube assembly. Development (Cambridge, England). 134(13):2379-2386
Glickman, N.S., Kimmel, C.B., Jones, M.A., and Adams, R.J. (2003) Shaping the zebrafish notochord. Development (Cambridge, England). 130(5):873-887
Glickman, N.S. and Yelon, D. (2002) Cardiac development in zebrafish: coordination of form and function. Seminars in cell & developmental biology. 13(6):507-513
Glickman, N.S. and Yelon, D. (2002) Analysis of the cellular behaviors driving cardiac fusion in zebrafish. Developmental Biology. 247(2):473

Larsen E., Lee, T. and Glickman, N. (1996). Antenna to leg transformation: dynamics of developmental competence. Dev Genet. 19(4):333-9.