PUBLICATION
The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)
- Authors
- Chang, W.J., Horng, J.L., Yan, J.J., Hsiao, C.D., and Hwang, P.P.
- ID
- ZDB-PUB-090217-5
- Date
- 2009
- Source
- American journal of physiology. Regulatory, integrative and comparative physiology 296(4): R1192-R1201 (Journal)
- Registered Authors
- Horng, Jiun-Lin, Hsiao, Chung-Der, Hwang, Pung Pung
- Keywords
- none
- MeSH Terms
-
- Acid-Base Equilibrium*
- Adaptation, Physiological
- Animals
- Cell Differentiation*
- Cloning, Molecular
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Enzymologic
- Gene Knockdown Techniques
- Gills/embryology
- Gills/enzymology*
- In Situ Hybridization
- RNA, Messenger/metabolism
- Skin/embryology
- Skin/enzymology*
- Sodium-Potassium-Exchanging ATPase/metabolism
- Time Factors
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Vacuolar Proton-Translocating ATPases/genetics
- Vacuolar Proton-Translocating ATPases/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 19193938 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
Citation
Chang, W.J., Horng, J.L., Yan, J.J., Hsiao, C.D., and Hwang, P.P. (2009) The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio). American journal of physiology. Regulatory, integrative and comparative physiology. 296(4):R1192-R1201.
Abstract
H(+)-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na(+) uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm-2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h post-fertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole-mount in situ hybridization, zgcm2 messenger (m)RNA signals were found in a group of cells on the zebrafish yolk-sac surface initially in the tail-bud stage (10 hpf); they had disappeared at 36 hpf, and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific co-localization of zgcm2 mRNA in HR cells in zebrafish embryons. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H(+) activity at the apical surface of HR cells, but it did not affect the occurrence of Na(+)-K(+)-ATPase-rich (NaR)cells. A decrease in zatp6v1a (the H(+)-ATPase subunit A) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 d after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 d post transfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation. Key words: acid secretion, ionocytes, ion regulation, gills.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping