PUBLICATION
Cloning and functional analysis of the proximal promoter region of the three GnRH genes from the silver sea bream (Sparus sarba)
- Authors
- Hu, S.Y., Chen, M.H., Lin, Y.C., Lin, G.H., Gong, H.Y., and Wu, J.L.
- ID
- ZDB-PUB-080922-2
- Date
- 2008
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 151(4): 373-380 (Journal)
- Registered Authors
- Chen, Mark Hung-Chih, Gong, Hong-Yi, Lin, Gen-Hwa, Wu, Jen-Leih
- Keywords
- gonadotropin-releasing hormone, promoter analysis, silver sea bream
- MeSH Terms
-
- Animals
- Brain Chemistry*
- Cloning, Molecular
- Conserved Sequence
- Gonadotropin-Releasing Hormone/genetics*
- Mesencephalon
- Olfactory Bulb
- Preoptic Area
- Promoter Regions, Genetic/genetics*
- Prosencephalon
- Protein Isoforms
- Sea Bream
- PubMed
- 18790071 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Hu, S.Y., Chen, M.H., Lin, Y.C., Lin, G.H., Gong, H.Y., and Wu, J.L. (2008) Cloning and functional analysis of the proximal promoter region of the three GnRH genes from the silver sea bream (Sparus sarba). Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 151(4):373-380.
Abstract
Gonadotropin-releasing hormone (GnRH) is a neuropeptide that plays a major role in releasing pituitary gonadotropin and controlling vertebrate reproduction. In this study, three GnRH cDNAs, GnRH-I (sbGnRH; 348 bp), GnRH-II (cGnRH-II; 557 bp), and GnRH-III (sGnRH; 483 bp), were cloned from the brain of the silver sea bream (Sparus sarba). In order to understand how the expression of the GnRH isoforms was regulated in the brain, the promoter of each gene was cloned and analyzed. We found regulatory motifs in the promoters that were conserved in the GnRH promoters of tilapia and zebrafish, suggesting that these motifs play a critical role in GnRH regulation. We performed functional analyses and examined tissue-specific expression for each GnRH promoter using EGFP reporter fusions in zebrafish. The GnRH-I promoter was active in the forebrain area, including the olfactory bulb-terminal nerve area and peripheral preoptic areas; the GnRH-II promoter was active in the midbrain; and the GnRH-III promoter was active in the olfactory bulb. These results show that the GnRH promoters of the silver sea bream GnRH genes exhibit tissue-specific activity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping