PUBLICATION

Direct and indirect roles for Nodal signaling in two axis conversions during asymmetric morphogenesis of the zebrafish heart

Authors
Baker, K., Holtzman, N.G., and Burdine, R.D.
ID
ZDB-PUB-080915-18
Date
2008
Source
Proceedings of the National Academy of Sciences of the United States of America   105(37): 13924-13929 (Journal)
Registered Authors
Baker, Kari, Burdine, Rebecca, Holtzman, Nathalia Glickman
Keywords
asymmetry, southpaw, switch hitter, cardiac, rotation
MeSH Terms
  • Left-Right Determination Factors
  • Nodal Protein
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism*
  • Signal Transduction*
  • Heart/embryology*
  • Myocardium/cytology
  • Myocardium/metabolism*
  • Gene Expression Regulation, Developmental
  • Animals
  • Cell Movement
  • Body Patterning*
(all 15)
PubMed
18784369 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
The Nodal signaling pathway plays a conserved role in determining left-sided identity in vertebrates with this early left-right (L/R) patterning influencing the asymmetric development and placement of visceral organs. We have studied the role of Nodal signaling in asymmetric cardiac morphogenesis in zebrafish and describe two distinct rotations occurring within the heart. The first is driven by an asymmetric migration of myocardial cells during cardiac jogging, resulting in the conversion of the L/R axis to the dorsal-ventral (D/V) axis of the linear heart. This first rotation is directly influenced by the laterality of asymmetric gene expression. The second rotation occurs before cardiac looping and positions the original left cells exposed to Nodal signaling back to the left of the wild-type (WT) heart by 48 hours postfertilization (hpf). The direction of this second rotation is determined by the laterality of cardiac jogging and is not directly influenced by asymmetric gene expression. Finally, we have identified a role for Nodal signaling in biasing the location of the inner ventricular and outer atrial curvature formations. These results suggest that Nodal signaling directs asymmetric cardiac morphogenesis through establishing and subsequently reinforcing laterality information over the course of cardiac development.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPtwu34Tgstandard conditions14-19 somites to 20-25 somitesIFL
EGFPtwu34Tg + MO1-dnaaf1standard conditions14-19 somites to 20-25 somitesIFL
lft2WTstandard conditions20-25 somitesISH
lft2WTstandard conditions20-25 somitesISH
lft2WTstandard conditions26+ somitesISH
lft2WTstandard conditionsPrim-5ISH
lft2WTstandard conditionsPrim-5ISH
lft2WT + MO1-dnaaf1standard conditions26+ somitesISH
lft2WT + MO1-dnaaf1standard conditions20-25 somitesISH
lft2WT + MO1-dnaaf1standard conditions20-25 somitesISH
1 - 10 of 15
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Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-dnaaf1standard conditions20-25 somites
WT + MO1-dnaaf1standard conditions20-25 somites
WT + MO1-dnaaf1standard conditions20-25 somites
WT + MO1-dnaaf1standard conditions26+ somites
WT + MO1-dnaaf1standard conditionsPrim-5
WT + MO1-dnaaf1standard conditionsPrim-5
WT + MO1-dnaaf1standard conditionsPrim-5
twu34Tg + MO1-dnaaf1standard conditions14-19 somites to 20-25 somites
1 - 8 of 8
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sk74TgTransgenic Insertion
    twu34TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      dnaaf1MO1-dnaaf1MRPHLNO
      spawMO3-spawMRPHLNO
      1 - 2 of 2
      Show
      Fish
      Fish
      sk74Tg
      twu34Tg
      twu34Tg + MO1-dnaaf1
      WT
      WT + MO1-dnaaf1
      1 - 5 of 5
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      EGFPEFGEGFP
      1 - 2 of 2
      Show
      Mapping
      No data available
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      Citation
      Baker, K., Holtzman, N.G., and Burdine, R.D. (2008) Direct and indirect roles for Nodal signaling in two axis conversions during asymmetric morphogenesis of the zebrafish heart. Proceedings of the National Academy of Sciences of the United States of America. 105(37):13924-13929.