ZFIN ID: ZDB-PUB-080326-25
Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish
Langenau, D.M., Keefe, M.D., Storer, N.Y., Jette, C.A., Smith, A.C., Ceol, C.J., Bourque, C., Look, A.T., and Zon, L.I.
Date: 2008
Source: Oncogene   27(30): 4242-4248 (Journal)
Registered Authors: Bourque, Caitlin, Ceol, Craig, Jette, Cicely A., Keefe, Matthew, Langenau, David, Look, A. Thomas, Smith, Amanda, Storer, Narie, Zon, Leonard I.
Keywords: Myc, Ras, heat-shock, T-cell acute lymphoblastic leukemia, rhabdomyosarcoma
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Transformation, Neoplastic/genetics*
  • Cell Transformation, Neoplastic/radiation effects*
  • Cleavage Stage, Ovum
  • DNA-Binding Proteins/administration & dosage
  • DNA-Binding Proteins/genetics
  • Embryo, Nonmammalian
  • Gene Transfer Techniques*
  • Genes, bcl-2
  • Genes, myc
  • Genes, p53
  • Green Fluorescent Proteins/administration & dosage
  • Green Fluorescent Proteins/genetics
  • Luminescent Proteins/administration & dosage
  • Luminescent Proteins/genetics
  • Microinjections/methods*
  • Mutant Proteins/genetics
  • Neoplasms, Radiation-Induced/genetics*
  • Nuclear Proteins/administration & dosage
  • Nuclear Proteins/genetics
  • Precursor T-Cell Lymphoblastic Leukemia-Lymphoma/genetics
  • Proto-Oncogene Proteins/genetics
  • Transgenes
  • Zebrafish/embryology
  • ras Proteins/genetics
PubMed: 18345029 Full text @ Oncogene
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.