PUBLICATION

Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis

Authors
White, R.M., Sessa, A., Burke, C., Bowman, T., LeBlanc, J., Ceol, C. Bourque, C., Dovey, M., Goessling, W., Burns, C.E., and Zon, L.I.
ID
ZDB-PUB-080324-7
Date
2008
Source
Cell Stem Cell   2(2): 183-189 (Journal)
Registered Authors
Bowman, Teresa, Burke, Christopher, Burns (Erter), Caroline, Ceol, Craig, Dovey, Michael, Goessling, Wolfram, LeBlanc, Jocelyn, Sessa, Anna, White, Richard M., Zon, Leonard I.
Keywords
STEMCELL, DEVBIO
MeSH Terms
  • Albinism/genetics
  • Animals
  • Flow Cytometry
  • Light
  • Models, Animal*
  • Neoplasm Transplantation
  • Pigmentation/genetics*
  • Stem Cell Transplantation*
  • Stem Cells/ultrastructure*
  • Zebrafish*
PubMed
18371439 Full text @ Cell Stem Cell
Abstract
The zebrafish is a useful model for understanding normal and cancer stem cells, but analysis has been limited to embryogenesis due to the opacity of the adult fish. To address this, we have created a transparent adult zebrafish in which we transplanted either hematopoietic stem/progenitor cells or tumor cells. In a hematopoiesis radiation recovery assay, transplantation of GFP-labeled marrow cells allowed for striking in vivo visual assessment of engraftment from 2 hr–5 weeks posttransplant. Using FACS analysis, both transparent and wild-type fish had equal engraftment, but this could only be visualized in the transparent recipient. In a tumor engraftment model, transplantation of RAS-melanoma cells allowed for visualization of tumor engraftment, proliferation, and distant metastases in as little as 5 days, which is not seen in wild-type recipients until 3 to 4 weeks. This transparent adult zebrafish serves as the ideal combination of both sensitivity and resolution for in vivo stem cell analyses.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes