PUBLICATION
Epidermal transient down-regulation of retinol-binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development
- Authors
- Tingaud-Sequeira, A., Forgue, J., Andre, M., and Babin, P.J.
- ID
- ZDB-PUB-060825-21
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(11): 3071-3079 (Journal)
- Registered Authors
- Babin, Patrick J.
- Keywords
- none
- MeSH Terms
-
- Carrier Proteins/analysis
- Carrier Proteins/genetics
- Apolipoproteins E/analysis
- Apolipoproteins E/genetics
- Larva/growth & development
- Larva/metabolism
- Transcription, Genetic/drug effects
- Zebrafish/genetics
- Zebrafish/growth & development*
- Morphogenesis/drug effects
- Morphogenesis/genetics*
- Receptors, Estrogen/analysis
- Receptors, Estrogen/genetics
- Animals
- Retinol-Binding Proteins, Plasma
- Zebrafish Proteins/analysis
- Zebrafish Proteins/genetics
- Epidermis/chemistry
- Epidermis/drug effects
- Epidermis/growth & development*
- Thiourea/analogs & derivatives
- Thiourea/pharmacology
- Gene Expression Regulation, Developmental*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Down-Regulation
- Up-Regulation
- PubMed
- 16929533 Full text @ Dev. Dyn.
- CTD
- 16929533
Citation
Tingaud-Sequeira, A., Forgue, J., Andre, M., and Babin, P.J. (2006) Epidermal transient down-regulation of retinol-binding protein 4 and mirror expression of apolipoprotein Eb and estrogen receptor 2a during zebrafish fin and scale development. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(11):3071-3079.
Abstract
Very little is known about the molecular control of skin patterning and scale morphogenesis in teleost fish. We have found radially symmetrical epidermal placodes with down-regulation of retinol-binding protein 4 (rbp4) expression during the initial paired fin and scale morphogenesis in zebrafish (Danio rerio). This finding may be related to changes in keratinocyte cytodifferentiation and/or the integument retinoid metabolism. rbp4 transcripts are expressed afterward in the central epidermis of the scale papilla and gradually extend to the epidermis, covering the growing scale, whereas no transcripts were detected in posterior margin epidermis. In contrast, induction of apolipoprotein Eb (apoeb) and up-regulation of estrogen receptor 2a (esr2a) transcripts were observed in the epidermis at initiator sites of zebrafish ectodermal/dermal appendage morphogenesis. This expression was maintained in the posterior margin epidermis of the formed scales. esr2a was also strongly expressed in neuromasts, whereas no rbp4 and apoeb transcripts were detected in these mechanosensory structures. The observed epidermal molecular events suggest that epidermis patterning is due to an activator-inhibitor mechanism operational at epidermal-dermal interaction sites. rbp4 transcript expression was also strongly down-regulated by 1-phenyl-2-thio-urea (PTU). As this inhibitor is commonly used to block obscuring pigmentation during in situ hybridization studies, this finding suggests that PTU should be used with caution, particularly in studying skin development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping