PUBLICATION

Cloning and embryonic expression of zebrafish PLAG genes

Authors

Pendeville, H., Peers, B., Kas, K., and Voz, M.L.

ID

ZDB-PUB-060105-15

Date

2006

Source

Gene expression patterns : GEP 6(3): 267-276 (Journal)

Registered Authors

Peers, Bernard, Pendeville-Samain, Hélène, Voz, Marianne

Keywords

PLAG, Zebrafish, Proliferation, Oncogene, Orthologue, Expression pattern

MeSH Terms

Embryo, Nonmammalian/embryology
Embryo, Nonmammalian/metabolism
Molecular Sequence Data
Brain/embryology
Brain/metabolism
Phylogeny
Amino Acid Motifs
Transcription Factors/genetics
Animals
Cloning, Molecular*
Immunohistochemistry
In Situ Hybridization
Humans
Protein Structure, Tertiary
Conserved Sequence
Sequence Homology, Amino Acid
Zebrafish/embryology*
Zebrafish/genetics*
Zebrafish/metabolism
Amino Acid Sequence
Zebrafish Proteins/chemistry
Zebrafish Proteins/genetics*
Gene Expression Regulation, Developmental*
Reverse Transcriptase Polymerase Chain Reaction
Synteny

(all 25)

PubMed

16378757 Full text @ Gene Expr. Patterns

Abstract

PLAG transcription factors play important roles in oncogenesis. To date three members of this subfamily of zinc finger proteins have been identified in humans and mice: PLAG1, PLAGL1 and PLAGL2. In this study, we identified zebrafish orthologs of PLAG1 and PLAGL2 and a novel member of this family, PLAGX. We examined the temporal expression of these three genes by quantitative real time RT-PCR and found that all three genes are maternally provided, expressed at low level during early somitogenesis and, during late somitogenesis and beyond, PLAG expression increases to reach a plateau level around 5 dpf. Whole mount in situ experiments revealed that PLAG1, PLAGL2 and PLAGX display a similar pattern of expression characterized by a low ubiquitous expression overcame by high expression in some restricted compartments such as the ventricular zone of the brain, the pectoral fin buds, the developing pharyngeal arches and the axial vasculature. We show that this pattern resembles the one observed for the proliferative marker PCNA, suggesting that the PLAG genes are expressed more strongly in zones of active proliferation. This hypothesis was proven for the ventricular zone shown to be a highly proliferative zone using the anti-phosphohistone H3 antibody that detects cells in mitosis.

Genes / Markers

Figures