PUBLICATION
Cloning and embryonic expression of zebrafish PLAG genes
- Authors
- Pendeville, H., Peers, B., Kas, K., and Voz, M.L.
- ID
- ZDB-PUB-060105-15
- Date
- 2006
- Source
- Gene expression patterns : GEP 6(3): 267-276 (Journal)
- Registered Authors
- Peers, Bernard, Pendeville-Samain, Hélène, Voz, Marianne
- Keywords
- PLAG, Zebrafish, Proliferation, Oncogene, Orthologue, Expression pattern
- MeSH Terms
-
- Amino Acid Motifs
- Amino Acid Sequence
- Animals
- Brain/embryology
- Brain/metabolism
- Cloning, Molecular*
- Conserved Sequence
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Molecular Sequence Data
- Phylogeny
- Protein Structure, Tertiary
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Synteny
- Transcription Factors/genetics
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish/metabolism
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- PubMed
- 16378757 Full text @ Gene Expr. Patterns
Citation
Pendeville, H., Peers, B., Kas, K., and Voz, M.L. (2006) Cloning and embryonic expression of zebrafish PLAG genes. Gene expression patterns : GEP. 6(3):267-276.
Abstract
PLAG transcription factors play important roles in oncogenesis. To date three members of this subfamily of zinc finger proteins have been identified in humans and mice: PLAG1, PLAGL1 and PLAGL2. In this study, we identified zebrafish orthologs of PLAG1 and PLAGL2 and a novel member of this family, PLAGX. We examined the temporal expression of these three genes by quantitative real time RT-PCR and found that all three genes are maternally provided, expressed at low level during early somitogenesis and, during late somitogenesis and beyond, PLAG expression increases to reach a plateau level around 5 dpf. Whole mount in situ experiments revealed that PLAG1, PLAGL2 and PLAGX display a similar pattern of expression characterized by a low ubiquitous expression overcame by high expression in some restricted compartments such as the ventricular zone of the brain, the pectoral fin buds, the developing pharyngeal arches and the axial vasculature. We show that this pattern resembles the one observed for the proliferative marker PCNA, suggesting that the PLAG genes are expressed more strongly in zones of active proliferation. This hypothesis was proven for the ventricular zone shown to be a highly proliferative zone using the anti-phosphohistone H3 antibody that detects cells in mitosis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping