PUBLICATION

Variable Meis-dependence among paralog group-1 Hox proteins

Authors
Choe, S.K., and Sagerström, C.G.
ID
ZDB-PUB-050513-6
Date
2005
Source
Biochemical and Biophysical Research Communications   331(4): 1384-1391 (Journal)
Registered Authors
Choe, Seong-Kyu, Sagerström, Charles
Keywords
Activation domain; TALE protein; Homeodomain; Transcription; Cofactor; Branchiomotor neuron; Hindbrain; Rhombomere; Zebrafish
MeSH Terms
  • Animals
  • Base Sequence
  • DNA Primers
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Zebrafish
PubMed
15883028 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Optimal function of Hox transcription factors may require Meis and Pbx cofactors. Here we test the in vivo Meis-dependence of two zebrafish paralog group-1 (PG1) Hox proteins. Misexpression of Hoxb1a induces ectopic gene expression throughout the anterior nervous system, while Hoxb1b induces ectopic expression primarily in hindbrain rhombomere 2. These activities are drastically reduced when endogenous Meis function is disrupted, demonstrating that both proteins are Meis-dependent. Upon addition of Meis3, Hoxb1b mimics the more severe Hoxb1a phenotype, indicating that Hoxb1b requires higher Meis levels than Hoxb1a. Using chimeric proteins we map this difference to the N-terminus, which contains the transcription activation domain. Lastly, we demonstrate strong genetic interactions between meis and PG1 hox genes, as well as between meis and pbx genes, in vivo. Our results are consistent with PG1 hox genes requiring pbx and meis function in vivo and reveal that different Hox proteins have distinct Meis requirements.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes