ZFIN ID: ZDB-PUB-010423-6
Isolation and characterization of posteriorly restricted genes in the zebrafish gastrula
Sagerström, C.G., Kao, B.A., Lane, M.E., and Sive, H.
Date: 2001
Source: Developmental dynamics : an official publication of the American Association of Anatomists   220(4): 402-408 (Journal)
Registered Authors: Lane, Mary Ellen, Sagerström, Charles, Sive, Hazel
Keywords: subtractive hybridization; cloning; cDNA; patterning; ectoderm; hindbrain; zebrafish; zinc finger; homeobox; transcription factor; gene; Danio rerio; neural; axis
MeSH Terms:
  • Animals
  • Cloning, Molecular
  • DNA, Complementary/metabolism
  • Genetic Techniques*
  • Nucleic Acid Hybridization
  • Sequence Analysis, DNA
  • Time Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zinc Fingers
PubMed: 11307172 Full text @ Dev. Dyn.
In order to understand anteroposterior axis formation in vertebrates, we have used subtractive hybridization to clone genes expressed posteriorly in the zebrafish gastrula-stage embryo. Here we report the initial characterization of eight clones isolated from this screen. We find that all eight genes are expressed in posteriorly restricted domains, suggesting that they are involved in regulating posterior development during zebrafish embryogenesis