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Fig. 2 Galcb knockout zebrafish exhibits impaired locomotion behaviour. (A) Representative swim behaviour tracking for wild-type (WT) and galcb knockout (KO) zebrafish at 4 months post fertilization (mpf). Alterations of fish locomotion of galcb KO mutants versus WT siblings was recorded for 5 min from the side view of the tank and quantified by measuring the number of body rotation/min (B) and total distance moved (C) using the Ethovision software equipped with a colour camera (five animals/group). Data are the mean ± standard error of the mean. Student's t-test: *P < 0.05, **P < 0.01. (D) Time frame images from Supplementary Video 1 showing the swim behaviour of WT and galcb KO zebrafish at 4 mpf.