Figure 3

Figure 3

cnr1 antagonism leads to reduction in mature MCC population: (A) 28 ss WT, 0.02 mM AM-251 and 0.02 mM AM-281-treated embryos stained via WISH using the mature MCC marker odf3b and cetn4; scale bar = 50 μm; (B,C) number of odf3b⁺ and cetn4⁺ cells per nephron at 28 ss; (D) 24 ss WT, 0.02 mM AM-251 and 0.02 mM AM-281-treated embryos stained via WISH using the progenitor MCC marker pax2a and jag2b; scale bar = 50 μm; (E,F) number of pax2a⁺ and jag2b⁺ cells per nephron at 24 ss. Data presented on graphs are represented as mean ± SD;** p < 0.01 (ANOVA).