|
Fig. 2 Srrm4 is expressed in proliferating notch1a and differentiated elavl3 neurons during early brain development. a–d) Partial (20 μm depth) maximum intensity projections of srrm4 mRNA expression at 24 and 48 hpf. Dorsal view in (a) and sagittal view in (b) at 24 hpf. Dorsal view in (c) and sagittal view in (d) at 48 hpf. Anterior is to the left. Scale bars = 100 μm. e–e″) Single plane image of Mo of srrm4 co-labeled with notch1a mRNA at 48 hpf. e) srrm4 expression. e′) notch1a expression. e″) srrm4 and notch1a merged images. f–f″) Single plane image of Mo of srrm4 co-labeled with elavl3 mRNA at 48 hpf. f) srrm4 expression. f′) elavl3 expression. f″) srrm4 and elavl3 merged images. Areas of overlap, which appear yellow, indicate presumed co-localization. Scale bars = 50 μm. Cb, cerebellum; Di, diencephalon; Mo, medulla oblongata; Oe, olfactory epithelium; Ot, optic tectum; Te, telencephalon.