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Fig. 4 Dpy19l1l−/− mutants lack RF. (A) Confocal images showing the location of RF by immunostaining with SCO-spondin antibody (red) and cilia antibody (green) in control siblings and dpy19l1l−/− mutants at 36 hpf. Nuclei counterstained with DAPI. Yellow arrows indicate intact RF in controls. (B) External phenotypes of dpy19l1l−/− mutants injected with vehicle or dpy19l1l mRNA at 36 hpf. (C) Body axis angles of mutants in (B) quantified with ImageJ (means ± SEM; P values above bars, unpaired t test). (D) Confocal images of the reestablished RF (red) in dpy19l1l mRNA-rescued mutants at 36 hpf. Yellow arrows mark reestablished RF.