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Fig. 3 Metabolic phenotype following β-cell–specific deletion of Eif5a in adult mice. Eight-week-old male Eif5a-Δβ and control mice received intraperitoneal tamoxifen injections over 1 week and were allowed a week of recovery. Mice were fed a normal chow diet (NCD) for 5 weeks. N = 3 to 5 mice per group (each data point represents a different mouse). A, diagram showing the experimental design. B, quantitative RT–PCR for Eif5a1 and Eif5a2 transcripts from RNA from isolated islets. C, immunofluorescence and quantification of eIF5A (magenta) and insulin (green). Scale bar represents 100 μm. (D) Body weight. (E) Fat mass. (F) Lean mass. (G) Intraperitoneal glucose tolerance test (left panel) and area under the curve (right panel) of control and Eif5a-Δβ mice. Data are presented as mean ± SEM, and statistical significance was determined by an unpaired t test. eif5A, eukaryotic translation initiation factor 5A.