The expressions of some specification-related inner ear markers differed significantly between the sibling and dhx38−/− mutant embryos: (A) The expressions of two otic matrix protein markers were normal at 36 hpf in the sibling and dhx38−/− mutant embryos. Scale bar: 100 µm. (B) Two otic matrix protein markers displayed slight declines at 48 hpf and 56 hpf. Scale bar: 40 µm. (C) The in situ staining of markers for semicircular canal sensory cristae (bmp4), and saccular and utricular maculae marker (cahz), respectively. Scale bar: 40 µm. The epithelial pillar of the semicircular canal (ncs-1a), semicircular canal projections (dacha), and cilia (foxj1b). Scale bar: 100 µm. The red arrows indicate signal spots.
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