Fig.8

Fig.8

Offspring with low DHA status have dysregulated vision and stress response gene pathways. Morphologically normal 4 dpf larvae from wild-type mothers fed a DHA-sufficient diet (WT-SUFF) or elovl2^+/−mothers fed a DHA-deficient diet (elovl2-DEF) were used for bulk RNA-seq (n = 3 replicates/group, n = 25 pooled whole larvae/replicate). A: Principal component analysis shows that samples in the same group cluster together. B: Volcano plot of differentially expressed genes in elovl2-DEF compared to WT-SUFF (290 genes). Dashed lines mark thresholds used to assess statistical significance (FDR-adjusted P-value <0.1, ≥ 2 fold change). C: Significantly down- (maroon) and up- (coral) regulated gene pathways identified from a Metascape pathway enrichment analysis using the 290 DEGs (hypergeometric test with Benjamini-Hochberg procedure for multiple comparisons).