FIGURE 2

FIGURE 2

RNA sequencing analysis of differentially expressed genes (DEGs) between stage I oocytes with or without granulosa cells. (A) Schematic diagram of sequencing analysis. (B) Volcano plot of DEGs. “pure” sample versus “mixed” sample, upregulated and downregulated genes are indicated in red and blue, respectively. DEGs were identified based on the following criteria: p-value < 0.05 and log2|fold change| ≥ 1. (C) Gene Ontology (GO) enrichment analysis of differentially upregulated genes between stage I oocytes with or without granulosa cells. GO enrichment analysis was based on the following criteria: adjusted p-value < 0.05. (D)In situ hybridization (ISH) of mafba and DAPI staining of zebrafish ovary sections. Purple: RNA probe labeled mafba mRNA; Blue: DAPI labeled cell nuclei. Scale bar, 100 μm.