Fig. 4
Fig. 4 The cyp2r1 interruption caused the disturbance of folate pathway in zebrafish. The gene expression of slc19a1 (A), slc46a1 (B), and folr1 (C) in the intestine and liver of the cyp2r1−/− and wild-type zebrafish was analyzed. The gene expression of dhfr, and mthfr in the intestine (D) and liver (E) of the cyp2r1−/− and wild-type zebrafish was analyzed. F: the gene expression of fpgs and ggh in the liver was analyzed. G: the folate content in liver of the cyp2r1−/− and wild-type zebrafish was measured with microbiological assay. Data are presented as means ± SE (n ≥ 6 fish). *P < 0.05; **P < 0.01; ***P < 0.001; ns, no statistical significance.