FIGURE 4

FIGURE 4

Both osteoblast and osteoclast numbers increase upon exposure to 10 mT of ELF-EMFs in the fractured scale. (A) Representative results of flow cytometric analysis of cells from intact scales (left panel), unexposed (u.e.) fractured scales (middle panel), or fractured scales exposed to 10 mT ELF-EMFs (right panel) at 24 h.p.fr. After fracture stimulation, zebrafish were placed in the ring-shaped tank and were unexposed or exposed to 10 mT ELF-EMFs for 4 h. Zebrafish scales were extracted at 24 h.p.fr., and cell samples were prepared for flow cytometric analysis. Red and green gates show trap:GFP^–osterix:mCherry⁺ (“mCh⁺”) and trap:GFP^high (“GFP^high”) cells, respectively. (B,C) Percentage (B) and absolute number of mCh⁺ and GFP^high cells per scale (C) in intact scales, unexposed fractured scales (u.e. fractured), or fractured scales exposed to 10 mT ELF-EMFs (10 mT fractured). A total of 12 samples from 6 zebrafish were used in each condition. Asterisks indicate the p-value in one-way ANOVA with Dunnett’s test. Error bars, s.e.m.; *p < 0.05, **p < 0.01, ***p < 0.001.