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Fig. 1 The 3′ end sequences of mature mRNAs are shortened during early development in zebrafish and mice. (A) A schematic diagram of full-length zebrafish pou5f3 mRNA. The 3′ end sequences (100 nt) are presented at the bottom. Sequences in blue indicate an additional 3′ end. Sequences in green denote the poly(A) signal (PAS). ORF, open reading frame. (B) Sequence analysis of the 3′ ends of pou5f3 mRNA in immature (Im) and mature (M) oocytes and embryos at 0, 2, and 4 hpf. The lengths of 3′ ends (left) and poly(A) tails (right) are presented. Similar results were obtained from two independent experiments. (C) A schematic diagram of full-length mouse Pou5f1 mRNA. The 3′ end sequences (50 nt) are presented at the bottom. Sequences in green denote PAS. (D) PAT assay for mouse Pou5f1 mRNA in Im and M oocytes and early two-cell stage embryos (E2c). bp, base pair. Similar results were obtained from two independent experiments. Scale bar, 20 μm. (E) Sequence analysis of the 3′ ends of Pou5f1 mRNA in the PAT assay. The lengths of 3′ ends (left) and poly(A) tails (right) are presented. (F) Lengths of Pou5f1 poly(A) tails in the sequence analysis were determined (means ± SD). ****P < 0.0001 and **P < 0.01, Tukey’s multiple comparison test.