PUBLICATION
Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development
- Authors
- Takada, Y., Fierro, L., Sato, K., Sanada, T., Ishii, A., Yamamoto, T., Kotani, T.
- ID
- ZDB-PUB-231125-6
- Date
- 2023
- Source
- Science advances 9: eadg6532eadg6532 (Journal)
- Registered Authors
- Kotani, Tomoya
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryonic Development/genetics
- Mice
- Protein Biosynthesis*
- RNA Processing, Post-Transcriptional
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 38000026 Full text @ Sci Adv
Citation
Takada, Y., Fierro, L., Sato, K., Sanada, T., Ishii, A., Yamamoto, T., Kotani, T. (2023) Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development. Science advances. 9:eadg6532eadg6532.
Abstract
Eggs accumulate thousands of translationally repressed mRNAs that are translated into proteins after fertilization to direct diverse developmental processes. However, molecular mechanisms underlying the translation of stored mRNAs after fertilization remain unclear. Here, we report a previously unknown RNA processing of 3' end sequences of mature mRNAs that activates the translation of stored mRNAs. Specifically, 9 to 72 nucleotides at the 3' ends of zebrafish pou5f3 and mouse Pou5f1 mRNAs were deleted in the early stages of development. Reporter assays illustrated the effective translation of the truncated forms of mRNAs. Moreover, promotion and inhibition of the shortening of 3' ends accelerated and attenuated Pou5f3 accumulation, respectively, resulting in defective development. Identification of proteins binding to unprocessed and/or processed mRNAs revealed that mRNA shortening acts as molecular switches. Comprehensive analysis revealed that >250 mRNAs underwent this processing. Therefore, our results provide a molecular principle that triggers the translational activation and directs development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping