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Figure Caption
Fig. 3 Serotonergic neurons in the retina of larvae zebrafish at 8-dpf. (a, d) Immunostaining for 5-HT (green) in sagittal and horizontal sections of larval (8-dpf) retinae. 5-HT positive cells (green) are seen in the inner nuclear layer of the retina in both VPA-treated and control larvae in a similar distribution. (e) Quantification of the number of cells per section showed no statistical differences between treated animals and controls. Neurons were counterstained with HuD/HuC label (red). Data are expressed as mean ± SEM, n = 5 per group, *p-value <0.05. 5-HT, serotonin. Scale bar 50 μm.
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