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Fig. 2 Wild-type and mutant zebrafish Prc1l. (A) Top: Diagram of the predicted wild-type protein, depicting the conserved MAP65/Ase1/PRC1 domain. Middle: The lesion in the ENU-induced maternal-effect allele tmip4anua results in a truncation of the protein deleting a majority of this domain. Bottom: The CRISPR-Cas9-induced allele prc1lUW101 results in the deletion of two conserved amino acids (see Fig. S1B and Fig. S10) N-terminal to the conserved MAP65/Ase1/PRC1 domain. (B,C) Nucleotide sequence and translated amino acids showing the creation of a premature stop codon in tmip4anua (B) and the two-amino acid deletion in prc1lUW101 (C).