PUBLICATION
The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo
- Authors
- Nair, S., Welch, E.L., Moravec, C.E., Trevena, R.L., Hansen, C.L., Pelegri, F.
- ID
- ZDB-PUB-230216-30
- Date
- 2023
- Source
- Development (Cambridge, England) 150(4): (Journal)
- Registered Authors
- Nair, Sreelaja, Pelegri, Francisco
- Keywords
- Cytokinesis, Dorsal determinant, Microtubule reorganization, Midbody, Prc1, Zebrafish
- MeSH Terms
-
- Animals
- Cell Division
- Cytokinesis*/genetics
- Microtubule-Associated Proteins*/metabolism
- Microtubules/metabolism
- Zebrafish*
- PubMed
- 36789950 Full text @ Development
Citation
Nair, S., Welch, E.L., Moravec, C.E., Trevena, R.L., Hansen, C.L., Pelegri, F. (2023) The midbody component Prc1-like is required for microtubule reorganization during cytokinesis and dorsal determinant segregation in the early zebrafish embryo. Development (Cambridge, England). 150(4).
Abstract
We show that the zebrafish maternal-effect mutation too much information (tmi) corresponds to zebrafish prc1-like (prc1l), which encodes a member of the MAP65/Ase1/PRC1 family of microtubule-associated proteins. Embryos from tmi homozygous mutant mothers display cytokinesis defects in meiotic and mitotic divisions in the early embryo, indicating that Prc1l has a role in midbody formation during cell division at the egg-to-embryo transition. Unexpectedly, maternal Prc1l function is also essential for the reorganization of vegetal pole microtubules required for the segregation of dorsal determinants. Whereas Prc1 is widely regarded to crosslink microtubules in an antiparallel conformation, our studies provide evidence for an additional function of Prc1l in the bundling of parallel microtubules in the vegetal cortex of the early embryo during cortical rotation and prior to mitotic cycling. These findings highlight common yet distinct aspects of microtubule reorganization that occur during the egg-to-embryo transition, driven by maternal product for the midbody component Prc1l and required for embryonic cell division and pattern formation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping