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Deng et al., 2023 - Dynamic Regulation of brsk2 in the Social and Motor Development of Zebrafish: A Developmental Behavior Analysis

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Figure 2

Generation of brsk2ab mutant zebrafish. (A) structure modelling of zebrafish brsk2a and brsk2b protein. PK represents protein kinase domain; UBA represents ubiquitin-associated domain. (B) Two CRISPR/Cas9 guide RNAs were designed to target exon 10 in brsk2a and exon 4 in brsk2b, respectively. The mutations of brsk2a and brsk2b were verified by Sanger sequencing, and computational modelling showed the proteins were truncated through SWISS-MODEL tool (https://swissmodel.expasy.org/interactive, accessed on 12 July 2023). (C) To validate the knockout efficiency, total mRNA was extracted at 3 mpf followed by reverse transcription, and qRT-PCR was conducted to validate the expression level of brsk2a, and brsk2b was decreased in brsk2ab^−/− zebrafish (n = 3 for each genotype, brsk2a, p = 0.0007; brsk2b, p = 0.0004). Data are shown as mean ± SEM, *** p < 0.001.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.