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Figure 1—figure supplement 1. figure supplement 1.. characterization of the fluorescent reporter lines Tg(pcna:EGFP) and Tg(gfap:mCherry) in double transgenic animals. (A) Confocal images of immunohistochemistry against the proliferation marker PCNA (grey) and EGFP (blue) on retinal sections of Tg(pcna:EGFP);Tg(gfap:mCherry) animals. Schemes in the thumbnails on the top-right and top-left of the PCNA panels indicate the region of interest, either the central retina or the ciliary marginal zone (CMZ). N=3 fish. (B) Confocal images of immunohistochemistry detecting Zrf1-positive MG fibers (magenta) and mCherry on retinal sections of Tg(pcna:EGFP);Tg(gfap:mCherry) animals. N=3 fish. Scale bar: 30 µm for main panels, 10 µm for inserts.