ZFIN Image: Faini et al., 2023, Fig. 5

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Figure Caption

Fig. 5

Simulated temperature rise under cyclic and conventional holographic illumination.

a Temperature rise induced on an illuminated spot under conventional (black) and cyclic-illumination (blue), using a power per cell, 𝑃𝑠𝑡𝑑 $P_{s t d}$ , of 10 mW and 𝑃𝑐𝑦𝑐=𝐻⋅‾‾‾√𝑃𝑠𝑡𝑑 $P_{c y c} = \sqrt{H \cdot} P_{s t d}$ = 𝐻‾‾√⋅10 $\sqrt{H} \cdot 10$ mW, in conventional and cyclic-illumination, respectively; illumination t𝑑𝑤= $t_{d w} =$ 10 ms; H = 20; 50 µs illumination pulses; 1 ms per each cycle b Volumetric distribution of 160 spots (H = 20 holograms, m = 8 spot per hologram) uniformly distributed in a 350 × 350 × 100 µm³ volume. c Temperature rise induced on a central spot when 160 spots are illuminated as depicted in (b) under conventional (black) and cyclic-illumination (blue). Inset: Temperature rise induced on the central spot by the 159 neighboring spots. d, e Temperature rise for different spots density d distributed in the considered volume under conventional (d) and cyclic-illumination (e). f Maximum temperature rise for cyclic- and conventional-illumination as simulated in (d, e). t𝑑𝑤= $t_{d w} =$ 20 ms. Power per cell in conventional illumination 𝑃(𝑧)=10⋅𝑒𝑧/𝓁𝑠 $P (z) = 10 \cdot e^{z / l_{s}}$ mW; Power per cell in cyclic-illumination 𝑃(𝑧)=10⋅𝑒𝑧/𝓁𝑠⋅𝐻‾‾√ $P (z) = 10 \cdot e^{z / l_{s}} \cdot \sqrt{H}$ ; H = 20 in (b–d).

Acknowledgments

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