(A) Cardiac arrest rate. The heart area of 4-dpf Tg larvae expressing CoChR-tdTomato or ChrimsonR-tdTomato was irradiated with light (470 nm for CoChR; 520 nm for ChrimsonR) for 100 ms at a strength of 0.5, 0.2, or 0.05 mW/mm2. Six consecutive stimulation trials were analyzed for four rhodopsin-expressing larvae of each Tg line. (B, C) Latency to cardiac arrest (B) and time to resumption of heartbeats (C) after light stimulation with optogenetic tools. One-way ANOVA with Tukey’s post hoc test was used for statistical analysis. ns: not significant. Means and SEMs are indicated.
Data for Figure 5—figure supplement 2, cardiac arrest induced with CoChR and ChrimsonR.
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