ZFIN Image: Hagio et al., 2023, Figure 3—figure supplement 3

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Figure 3—figure supplement 3—source data 1. Optogenetic activation of hindbrain reticulospinal V2a neurons by <italic toggle='yes'>Co</italic>ChR and ChrimsonR.

(A) Light stimulation-dependent locomotion rates of 3-dpf Tg larvae expressing CoChR-tdTomato and ChrimsonR-tdTomato. The hindbrain area was irradiated with light (0.4 mW/mm²) with a wavelength of 470 nm (CoChR-tdTomato) or 520 nm (ChrimsonR-tdTomato) for 100 ms. Six consecutive stimulation trials were analyzed for eight rhodopsin-expressing and eight non-expressing (control) larvae of each Tg line. The average locomotion rates for each larva are shown. Wilcoxon rank-sum test (CoChR-tdTomato vs. control, p=0.000205; ChrimsonR vs. control, p=0.0509). (B–D) Latency (B), duration (C), and strength (D) of tail movements. Welch’s t-test (strength CoChR-tdTomato vs. ChrimsonR; p=0.00463). (E) Rate of locomotion induced with CoChR-tdTomato by light of various light intensities. **p<0.01, ***p<0.001, ns: not significant. Means and SEMs are indicated.

Figure 3—figure supplement 3—source data 1.Data for <xref rid='fig3s3' ref-type='fig'>Figure 3—figure supplement 3</xref>, optogenetic activation of V2a neurons by <italic toggle='yes'>Co</italic>ChR and ChrimsonR.

Acknowledgments

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