IMAGE

Fig. 3.

ID
ZDB-IMAGE-230707-31
Source
Figures for Zhang et al., 2023
Image
Figure Caption

Fig. 3.

Psap KO zebrafish exhibit impaired locomotion, storage material accumulation and myelin loss without significant loss of oligodendrocyte markers. (A) Representative images of a 4 mpf psap+63/+63 zebrafish and WT sibling. Length distributions of 4 mpf WT and psap KO zebrafish are shown in B. (B) Length measurements of 4 mpf psap+63/+63 zebrafish and WT siblings. n=17 WT, 17 psap+63/+63 fish. A decreased length was observed in nearly all psap+63/+63 and psap−14/−14 zebrafish for each generation examined. (C) Swim behavior tracking for 4 mpf psap−14/−14 and WT zebrafish. Disrupted locomotion was present in all psap+63/+63 and psap−14/−14 zebrafish for each generation examined. Representative data from five WT and six psap−14/−14 zebrafish. s, swim speed. Zebrafish were filmed from the side of a 2.75-l fish tank. The filming area encompasses the entire side view of the tank and is ∼28 cm in length and 13 cm in height. (D) Mean swim speed of 4 mpf WT, psap−14/+ and psap−14/−14 zebrafish. (E) H&E staining of 4 mpf psap+63/+63 and WT zebrafish liver sections. Representative data from three WT, one psap−14/−14 and three psap+63/+63 zebrafish. Arrows point to regions containing large, foamy cell clusters. (F) Transmission electron microscopy (TEM) of 4 mpf psap+63/+63 and WT zebrafish livers. The arrow indicates regions of storage material accumulation. Representative data from three WT and three psap+63/+63 zebrafish. (G) Black Gold II staining of frozen brain sections from 4 mpf psap+63/+63 zebrafish and WT siblings. Representative data from five WT and five psap+63/+63 zebrafish. Additional serial sections are in Fig. S5, and several brain sections from G are also reproduced among the sequential sections in Fig. S5. (H) TEM of optic nerve sections from 4 mpf psap+63/+63 zebrafish and WT siblings. Representative data from five WT and six psap+63/+63 zebrafish. (I) Schematic of the expression of stage-specific marker genes (top). qRT-PCR of major oligodendrocyte differentiation markers in 4 mpf psap+63/+63 and WT sibling zebrafish brains (bottom). Representative data from two independent datasets; n=4 WT and 4 psap+63/+63 for each dataset. (J) qRT-PCR of olig2, pdgfra and mbpa over the course of psap+63/+63 and WT sibling zebrafish brain development. Representative data from at least two independent datasets for each time point; n=3-5 WT and 3-5 psap+63/+63 for each dataset. Data show the mean±s.e.m. For B,D,I,J, two-tailed unpaired Student's t-test was used. *P<0.05; **P<0.01, ***P<0.001.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.