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Figure 1
Molecular characterization of D4 mutant embryos. (A) Western blot for Pank2 in mutant (D4) and control embryos (Ctl). Lysates from SH-SY5Y cells (Cells) were run as controls. (B) qPCR analysis on mutant and control embryos for pank2, coasy, pank1a, and pank1b. (C) Coenzyme A quantification by a fluorimetric assay. Data derived from the analysis of 20 embryos/group, with two biological replicates for Western blotting and at least three for qPCR and CoA quantification. ** p < 0.01; **** p < 0.0001, Student’s t-test.
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