ZFIN Image: Angom et al., 2021, Fig. 2

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Figure Caption

Fig. 2

LCC-09 inhibits LPS-induced inflammatory response in zebrafish. (A–D) Zebrafish (MPO;GFP) embryos at 48 hpf were treated with LCC-09 (5 μM) and DMSO as control for 3 h and then injected with LPS (0.4 mg/mL, 2 nL) through yolk sac. Images were collected 12 h after injection (B). Local accumulation of neutrophils was quantified using GFP fluorescent intensity and compared (C). Zebrafish survival was monitored (N=8 per group) (D). (E–G) LPS (0.4 mg/mL, 2 nL) was intravenously injected to the caudal vein of zebrafish (MPO;GFP) embryos at 48 hpf. Images were collected 6 h post-injection. Neutrophil accumulation in tail fins (outlined by yellow dotted dash lines) was counted and compared. Arrow denotes infiltration of GFP positive neutrophils induced by LPS injection. *p<0.05.

Acknowledgments

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