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Fig. 5
Median fin histology of adult wild-type and gdf5uu3703/uu3703 zebrafish reveals intact joints between distal and proximal radials and normal maturation of the skeletal elements. Sagittal sections from 60 dpf of wild-type (A,A,C,C′,E,E′) and gdf5uu3703/uu3703 (B,B′,D,D′,F,F′) zebrafish median fins stained with Hematoxylin and Eosin (HE). Anterior to the left, dorsal to the top. Dashed boxes in A-F mark magnified region in A′-F′. (A,A′,B,B′) Dorsal fin; (C,C′,D,D′) anal fin; (E,E′,F,F′) caudal fin. (A′-D′) White arrowheads indicate joint capsule, black arrowheads indicate articular cartilage. All joints are intact and do not display any differences when compared to wild-type. (E-F,E′-F′) Caudal fin skeletal elements display no apparent delay in chondrocyte maturation when compared to wild-type. Scale bars: 500 μm (A-F), 100 μm (A′-F′)
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