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Figure 6

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ZDB-IMAGE-220801-142
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Figures for Hawkey-Noble et al., 2022
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Figure 6

Calcein staining illustrating the impact of foxl1, foxc1a, and foxc1b lone and combined loss on axial skeleton development and calcification. (A) WT, foxl1-/-, foxc1a-/-, and foxc1b-/- embryos at 6 dpf showing normal formation of the vertebrae in foxl1 and foxc1b mutants. foxc1a-/- embryos did not develop or calcify vertebrae and had large abdomen cavity edemas that grew over time. (B) WT; foxc1a-/-; foxl1-/-, foxc1b-/-; foxl1-/-, and foxc1a-/-; foxc1b-/- embryos at 6 dpf. Double foxc1a-/-; foxl1-/- embryos exhibit larger edemas and a lack of most calcified structures at 6 dpf, including the operculum (OP). foxc1b-/-; foxl1-/- embryos had no significant change. foxc1a-/-; foxc1b-/- mutants elicited similar results as foxc1a-/-; foxl1-/- embryos, including edemas and lack of craniofacial calcification. (C) Boxplots illustrating the variability in the number of vertebrae calcified in foxl1-/-, foxc1a-/-, foxc1b-/-,and foxl1-/-; foxc1b-/- mutants in comparison with WT embryos at 6 dpf. Red arrows indicate primary centres of ossification within the vertebrae. Scale bars are 500 µm.

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