Fig. 3

Figure 3. Cell proliferation and cell death in the intestine after 6 weeks of HCC induction. (A) Representative images of immunofluorescent staining for PCNA in the three intestine segments. (B) Quantification of cell proliferation in the intestine. Percentage of PCNA⁺ cell counts compared to DAPI⁺ cell counts is presented. (C) Representative images of TUNEL staining, classified into four different patterns. (D) Quantification of cell death pattern in the intestine based on frequency. Scale bar in red 50 μm. IB: intestinal bulb; MI: mid-intestine; CI: caudal intestine. * p < 0.05, **** p < 0.0001.