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Fig. 2
Validation of segmentation accuracy. (A) Comparing manual measurements (gray dots) to automated FWHM (black dots) showed good agreement. White arrowheads indicate outliers caused by skewed cross-sectional intensity distributions. (B) Comparison of manual measurements to FWHM, after GF with thresholding and SE with thresholding in the CtA (C), MMCtA (D), PMBC (E), and BA (F). Figures C-F show the Kruskal-Wallis test results. (G) Average voxel error is independent of vessel diameter (averaged n = 6; 3 dpf embryos). (H) Visual comparison of original data to images after SE and segmentation using 3D rendering.
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