ZFIN Image: Kugler et al., 2022, Fig. 6

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Figure Caption

Fig. 6

Segmentation of different transgenic lines. (A-C) In all three double transgenics [(1) Tg(fli1a:eGFP)^y1, Tg(kdrl:HRAS-mCherry)^s916, (2) Tg(fli1a:CAAX-eGFP), Tg(kdrl:HRAS-mCherry)^s916, and (3) Tg(fli1a:LifeAct-mClover)^sh467, Tg(kdrl:HRAS-mCherry)^s916] nonvascular signal was observed in the fli1a-driven transgenic (arrowheads). (D-E) Segmentation results of the three double transgenics showed nonvascular signals to be enhanced and segmented in the transgenics under the fli1a promotor. (F) Vascular volume in Tg(fli1a:eGFP)^y1 was statistically significantly higher than in Tg(kdrl:HRAS-mCherry)^s916 (p < 0.0001; n = 21; paired t-test). (G) Vascular volume in Tg(fli1a:CAAX-eGFP) was statistically significantly higher than Tg(kdrl:HRAS-mCherry)^s916 (p < 0.0001; n = 17; paired t-test). (H) Vascular volume in Tg(fli1a:LifeAct-mClover)^sh467 was statistically significantly higher than Tg(kdrl:HRAS-mCherry)^s916 (p < 0.0001; n = 23; paired t-test).

Acknowledgments

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