(A) agrp expression analysis of CR and DIO fish brains as well as CG9 fish brains 1–2 weeks after the shift to the obesogenic environment. Expression is standardized to rps23; (B) calorically restricted fish are severely hyperphagic when exposed to ad libitum feeding conditions compared to the DIO group that has been raised in an obesogenic environment; (C) CG3 fish are hyperphagic before (CR) transfer to DIO conditions as well as at least one week after transfer (CG3); (D) The CG9 group is similarly hyperphagic before as well as at least one week after the transfer to DIO conditions. Pooled data of male and female fish, n of 4–10; error bars indicate STDEV, groups were compared with ANOVA followed by Tukey’s multiple comparisons test (A) or an unpaired t-test (C, D), significance is indicated as * p<0.05, ** p<0.01 and *** p<0.001.