Fig 1

Fig 1

Upper part: scheme of the crossings and timing of the heat shocks (HS). Lower part: A-E: FISH performed at 55 hpf with a sox4b probe on ascl1a^-/- or control sibling embryos heat-shocked at 36 and 46 hpf F-J: FISH performed at 96 hpf with the agr2 probe revealed in green and a mix of hormone probes (ghrelin (ghrl), peptide YYb (pyyb), glucagon-a (gcga) and somatostatin-2 (sst2)) revealed in red on ascl1a^-/- or control sibling embryos heat-shocked at 36, 46 and 56 hpf. The transgenic line used is indicated on the left part of the figure as well as the genotype of the larvae; the ascl1a^-/- larvae were identified by the loss of the pituitary prl expression (not shown). The pancreas is encircled while the location of gut, visualised with a DAPI staining (not shown), is delimited by dashed lines. K: Quantification of the number of ghrl+/pyyb+/gcga+/sst2+ EEC detected in conditions F to J counted under a fluorescent microscope. All views are ventral with the anterior part to the left and represent confocal projection images. Scale bar: 100 μm.