ZFIN Image: Hoyle et al., 2021, Fig. 4.

Image

Figure Caption

Fig. 4.

Loss of pthlha leads to dorsal expansion of entpd5a-expressing pre-hypertrophic chondrocytes in ch cartilage. (A-C) Live imaging of wild-type sox10:lyn-tdTomato;entpd5a:kaede double-transgenic embryos; ventral views. (D-F) Live imaging of pthlha CRISPR F0 sox10:lyn-tdTomato;entpd5a:kaede double-transgenic embryos; ventral views. (C,F) Optical slices showing the sox10:lyn-tdTomato chondrocyte doublets, magnified in insets. (A,C,D,F) Optical slices, (B,E) z-projections. White arrowheads indicate the position of the HZ. Blue arrowheads indicate the position of the secondary HZ. Green arrowheads indicate the position of chondrocyte doublets. (G) Quantification of entpd5a:kaede-labeled pre-hypertrophic chondrocytes (HCs) in the ch cartilage. (H) Quantification of chondrocyte doublets in the ch cartilage. BR3, branchiostegal ray 3; ch, ceratohyal; Ctrl, control; D, dorsal; pq, palatoquadrate; V, ventral. Scale bar: 50 μm (A-F).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development