ZFIN Image: Brombin et al., 2022, Figure 2

Image

Figure Caption

Figure 2

scRNA-seq identifies six distinct NC pigment cell lineage populations

(A) Schematic of the experimental protocol. GFP⁺ and/or mCherry⁺ cells were isolated from Tg(crestin:mCherry; mitfa:GFP) embryos at 24 hpf.

(B) UMAP of GFP⁺, mCherry⁺, and double-positive cells (n = 996 cells) obtained after Louvain clustering (dimensions, dims = 20, resolution = 0.5).

(C) Heatmap showing the average log₂ fold change expression of five selected genes per cluster identified in (B). The average log₂ fold change expression across the 6 clusters of sox10, crestin, mitfa, mCherry, and GFP expression levels are also presented for comparison.

(D) UMAP representations of (B) with color change from blue (negative) to red (positive) based on log₂ mRNA expression of pcna, twist1a, foxd3, aox5, tfec, mitfa, and tyrp1b.

(E) Pseudotime ordering of the cells in (B). Top: pseudocoloring based on pseudotime scores. Bottom: pseudocoloring based on cluster identity.

(F) RNA velocity analysis of the UMAP represented in (B) (top) and simplified representation (bottom).

See also Figures S1–S3 and Tables S1 and S2.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.