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Figures for Cavone et al., 2021
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Fig. 3 Tnf levels correlate with immune system-induced regenerative neurogenesis All photomicrographs are lateral views at 48 hpl (except A); asterisk indicates injury site; boxed areas are shown in higher magnification on the right. All qRT-PCR results are relative to unlesioned control, set to 1 and indicated by dashed red line; each dot represents one experiment. (A) Macrophages (mpeg1:GFP+) and Tnf protein are concentrated in the spinal injury site and close to (within 20 μm) motor neuron progenitor cells (olig2:DsRed+). A 4-dpf-larva is shown at 22 hpl (top: merge of all channels; middle: mpeg1:GFP; bottom: Tnf). See Figure S2A for separate channels and S2B for quantification. (B) qRT-PCR shows LPS increases and dexamethasone decreases tnfa expression (Incubation: −6 to 24 hpl for dexamethasone and 0 to 24 hpl for LPS; analysis at 24 hpl; Kruskal-Wallis test p = 0.0007; Dunn’s post-test: ∗p = 0.027; ∗∗∗p = 0.0003; n.s.: p = 0.687. (C) Dexamethasone decreases hdac1 mRNA expression after injury (incubation: −6 to 24 hpl; t test: ∗∗∗∗p = 0.0001). (D) Dexamethasone treatment reduces the number of proliferating motor neuron progenitor ERGs (olig2:GFP+/EdU+; incubation: dexamethasone: −6 to 48 hpl; EdU 0 to 48 hpl; t test: ∗p = 0.040). (E) LPS treatment increases hdac1 mRNA expression after injury (incubation: 0 to 24 hpl; t test: ∗p = 0.023). (F and G) LPS treatment increases the number of proliferating motor neuron progenitor ERGs (olig2:GFP+/EdU+) (F; t test, ∗∗∗∗p < 0.0001) and number of new motor neurons (G; mnx1:GFP+/EdU+; t test, ∗∗∗∗p < 0.0001); incubation: LPS: 0 to 48 hpl; EdU 0 to 48 hpl). Scale bars: 50 μm; 10 μm for insets. Data are represented as mean ± SEM (see also Figure S2; Tables S6–S8).

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Reprinted from Developmental Cell, 56(11), Cavone, L., McCann, T., Drake, L.K., Aguzzi, E.A., Oprişoreanu, A.M., Pedersen, E., Sandi, S., Selvarajah, J., Tsarouchas, T.M., Wehner, D., Keatinge, M., Mysiak, K.S., Henderson, B.E.P., Dobie, R., Henderson, N.C., Becker, T., Becker, C.G., A unique macrophage subpopulation signals directly to progenitor cells to promote regenerative neurogenesis in the zebrafish spinal cord, 1617-1630.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell