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Publication: Jimenez et al., 2021 - Vestibular and Auditory Hair Cell Regeneration Following Targeted Ablation of Hair Cells With Diphtheria Toxin in Zebrafish
All Figures: Figures for Jimenez et al., 2021

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FIGURE 2

Tg(myo6b:hDTR) larval zebrafish show hair cell loss and regeneration in lateral line neuromasts after in vivo DT treatment. (A) Schematic representation of the larval DT exposure treatment. (B) Wild-type (WT) and Tg(myo6b:hDTR) (hDTR) larvae were exposed to three concentrations of DT (0.5, 1, and 1.5 μg/mL) for various durations (3, 6, 8, 10, and 12 h). Neuromast viability was monitored daily by YO-PRO-1 labeling 0 to 72 h post-incubation. Shown is the quantification of YO-PRO-1 labeled hair cells. The values are represented as the mean ± SEM from eight fish. (C) Regeneration time-course showing P1–P4 over three days of recovery. Larvae were exposed to 1 μg/mL of DT for 6 h. Scale bar 100 μm.

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