ZFIN Image: Hung et al., 2021, Figure 2

Image

Figure Caption

Figure 2 Bad knockdown can enhance p53/caspase-8 death signaling. (A) Identification of Bad knockdown by specific morpholino (Bad-MO) at 8 hpf via Western blot analysis. (B,C) TUNEL-positive spot staining (indicated by arrows) at 8 hpf with Bad knockdown by Bad-MO. The Bad-MO group shows more signal than the Bad-M5 (Control group). TUNEL-positive spots within the embryo were counted and are shown in (C). The DNase I treatment group was used as a positive control. (D,E) Analysis of apoptotic-related genes during development and cell-death-related genes by qRT-PCR at 8 hpf with Bad knockdown. All data were analyzed using either paired or unpaired Student’s t-tests, as appropriate. TUNEL-positive cells under the fluorescence microscope are considered apoptotic.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.